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A quick RNA mini-prep for Neurospora mycelial cultures

is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora

Pollen Development in Anthers of Arabidopsis

in Petri dishes on a layer of sterilized superfine grade Perlite pre-wetted with Gamborg's B5 Medium (see Growing Arabidopsis protocol). 2. Incubate in a growth chamber at 19-23°C under cool white fluorescent light (1500 to 2200 lux).

E-Z 96® Viral RNA Protocol with Centrifugation

. 3. Pipet 500 ul QVL lysis buffer into the each well of the 96-well plate. Keep the microplate flat on the bench, shake vigorously back and forth for 30 seconds. Rotate the plate 90 and shake the plate for another 30 secondsB. Note: Add 20 ul