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文献和实验酵母染色体沉淀分析方法Yeast Chromatin Immunoprecipitation (ChIP) Assay
Needles (21-gauge [hot] and 26-gauge) Rotator Siliconized microcentrifuge tubes (seven sets with caps; one set without caps; labeled) Sonicator with microtip
=> Dechorinated zebrafish embryos. Add embryos to siliconized microcentrifuge tubes (no more than 100 per tube). Remove excess H2O. Gently fix by adding ice cold 4 paraformaldehyde in PBS and incubating on ice for ~30 minutes. (Fixing time
Nested RT-PCR for Hepatitis C from Paraffin Sections
RNA Extraction from Histologic Sections Unstained 4 µm thick sections of formalin fixed paraffin embedded liver biopsies were transferred from glass slides to 1.5 ml non-siliconized microcentrifuge tubes containing 200µl of TRIzol (Gibco
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