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文献和实验Unlabeled carboxylic acid substrate (e.g., 1 mM hydrocinnamic acid) [γ‐33 P]ATP of known concentration and specific activity (e.g., 10 mM, 6.8 cpm/pmol
Phosphoamino acid analysis:Mark Kamps's method
more than 3 µl is tedious,so don't use much H20.On the other hand,I wouldn't use much less than 6 µl so as to have good recovery. 7.Spot 1 µl PAA standards (about 0.3 µg each of PSer,PThr,and PTyr)on a cellulose thin layer chromatography plate and then spot
10ul Add 1ul of 4% Formic Acid and incubate for 25 min at 37℃ During this incubation, add 15 ul of stock piperidine to 135 ul of water to prepare a 1M piperidine solution. Place the tube containing the formic acid reaction on ice, add all 150
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