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文献和实验Preparation of total RNA from whole blood collected in PAXgene tubes
has been incubated in the PAXgene Blood RNA Tube for a minimum of 2 h at room temperature, in order to achieve complete lysis.Preheat a water bath or heat block (for microcentrifuge tubes) to 55℃, during first spin.We spin at 3750 rpm in a standard table
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
15 ml microcentrifuge tubes 3. Water baths preset at 37°C 4. Paper towels 实验步骤 1. Add one volume of whole blood (or bone marrow) to a nuclease-free 50 ml centrifuge tube containing 3 volume of Buffer ERL. Mix
them to by e-mail Tubes: 2-ml tubes with writing area that fit 100/2" box (Sarstedt #72.694.006) Labels : You will receive printed out labels for 6 sets of tubes from Stanford . If you start freezing before these are available, label each tube
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