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文献和实验The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3. A 5-primer with the minimum
The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3. A 5-primer with the minimum promoter sequences may be used
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
with the reagent. As a positive control, use cells set aside at the last step of the reprogramming procedure. b. Carry out a reverse transcription reaction using 1 µg of RNA (from step 1, above) and the SuperScript® VILO™ cDNA Synthesis Kit following
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