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文献和实验Clean-up Spin Protocol For DNA fragments (30bp-100 bp) 1) Add 3 x volume of buffer P3, 3 x volume of isopropanol to the enzymatic reaction and mix throughly with pipetting or vortex. The maximum volume of the reaction can be processed
Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors
with downstream applications. 13. Place column into a clean 1.5 mL microcentrifuge tube (not supplied). To elute DNA add 30 ìL-50 ìL of Elution Buffer (10 mM Tris buffer, pH 8.5) directly onto the center of HiBind matrix® . Incubate at 65EC
tube. To elute DNA, add 50 %26micro;l Buffer EB to the centre of each QIAprep column, let stand for 1 min, and centrifuge for 1 min. 6. Transfer the eluate to a clean labelled eppendorf tube-store on ice or at -20℃. Appendix DNA concentration
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