QIAGEN凯杰 代理商|205311 QuantiTect

Miniprep/Qiagen kit

  Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.

QIAGEN Plasmid plus Kit——超快纯化多至10 mg转染级别纯质粒

      ♦利用真空方式超快速进行质粒纯化，碱裂解步骤后，利用QIAfilter无须离心即可澄清裂解液，然后离心柱安置于真空底座（例如：QIAvac 24 Plus 见图1）上进行DNA的洗涤和洗脱。Midi and Maxi kit 20 分钟内可纯化24 个样本Mega和Giga Kit可分别在40，50分钟内处理12个样本。 ♦QIAGEN Plasmid plus Midi/Maxi Kit 提供优化的高产量protocol 并采取高洗脱体积，确保纯化得到高产量质粒

Large Scale Plasmid Preps: Qiagen/Cesium Method

: Qiagen Maxi Prep kit TE, pH8 Cesium Chloride CsCl/TE solution (d = 1.55 g/mL) 30 gm CsCl + 33 mL TE, check density Ethidium bromide 1 mg/mL Absolute ethanol 70% ethanol 3M NaOAc