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文献和实验Isolate It All:siRNA • miRNA • Total RNA • Native Protein
was adapted and combined with the advantages of the PARIS Kit , the first commercially available kit designed to isolate both RNA and native protein from the same sample [2]. The result is a versatile kit for quantitative recovery of native protein and all RNA
Native Chromatin Preparation and Illumina/Solexa Library Construction
carried over through protein-protein interactions. The protocol includes purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP
v468 Chapter 9 自身启动子报告基因验证转录靶点
/protein is to validate that the regulation through b-catenin occursat the level of transcription. One way to confirm this is to establishreporter constructs containing the native promoter regionof the putative target gene. Usage of reporter genes
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