- 询价
- AB1591P
- 2026年05月21日
- ELISA;Immunoprecipitation
- Rabbit
- Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
ELISA;Immunoprecipitation
- 宿主：
Rabbit
- 抗原来源：
0
- 适应物种：
Mouse;Rat
- 库存：
大量
- 是否单克隆：
2
- 规格：
50 μg
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | ELISA, IH, IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Dopamine Transporter
Background Information:
Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson's disease, Tourette's disease, schizophrenia, and addiction. It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm.
Alternate Names:
DAT
Specificity:
Recognizes Dopamine transporter (DAT). By Western blot the antibody recognizes a broad 80 kDa band in rat striatum (Lew et al. 1992; Freed et al. 1995; Vaughan et al. 1996) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.
Key Applications:
- ELISA
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Application Notes:
Western blot: 1-10 μg/mL using ECL. An antibody raised against a similar peptide has been shown to react with a broad 75-80 kDa band in rat striatum (Hastrup et al., 2001) and not cerebellum. Rat DAT contains various carbohydrate moieties and neuraminidase and N-Glycanase treatment reduces its molecular weight to about 55 kDa.
Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001)
Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: 2-10 μg of antibody (Chang et al., 2001)
Immunohistochemistry: 1:250-1:500 on frozen sections of tissues fixed with 4% paraformaldehyde (Chang et al., 2001; Ourednik, et al., 2002). 0.05% trypsin for 10-15min has been found effective in exposing the epitope in some preparations.
ELISA: 0.5-1.0 μg/mL using 1 μg/mL of DAT control peptide (Catalog Number AG296) per well.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Mouse
- Rat
Immunogen:
An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (Kilty et al. 1991; Giros et al. 1991; Shimada et al. 1991; Kitayma 1992). This region is presumably the cytoplasmic domain.
Molecular Weight:
80 kDa
Control:
Rat brain tissue
Presentation:
Affinity Purified immunoglobulin. Liquid in PBS containing 0.1% BSA
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Gene Symbol:
- SLC6A3
- ADHD
- DAT
- DAT1
- ADD
Antibody Category:
Neuroscience
Antibody Sub-Category:
- Ion Channels & Transporters
- Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Affinity Purified
Host:
Rabbit
Concentration:
500 μg/mL
Antibody Type:
Polyclonal Antibody
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
An 18 amino acid peptide sequence near the NH2-terminus of rat brain dopamine transporter coupled to KLH (Kilty et al. 1991; Giros et al. 1991; Shimada et al. 1991; Kitayma 1992). This region is presumably the cytoplasmic domain. 详细描述见链接：http://www.millipore.com/catalogue/item/AB1591P
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Anti-Dopamine Transporter 兔多克隆 亲和纯化 小鼠/大鼠 ELISA/IP/IH/WB
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