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- CWSS73SB1
- 2025年08月07日
- Food and Beverage;Beer
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Bevigard-M Cartridge FiltersUsed in final stage prefiltration applications, these versatile surface-type products retain contaminant on the membrane. Used as final filter for bioburden reduction, they offer an excellent efficiency of microorganism removal. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/CWSS73SB1
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文献和实验Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
. Put the folded filter onto a 50 ml tube or equilibrated Nucleobond AX cartridge, wet it with 0.5 - 1.0 ml of water and fill it with the cooled lysate. Collect the cleared flowthrough. These filters are suitable for plasmid isolation with Nucleobond AX 100
: Add 1 mL of 0.6 M bromoacetic acid (0.6 mmol) in DMF and 86 μL of N,N'-diisopropylcarbodiimide (0.93 equivalent, 0.56 mmol). Incubate with gentle bubbling for 30 minutes, then drain and rinse with 2 mL of DMF (repeat 4x). 6)
2 O for 1 hr. Fix in 1% glutaraldhyde/0.5 M sodium acetate for 30-45 min. Wash in diH 2 O, three times for 15 min each. Add Gel-Code Blue Stain reagent (Pierce, #24592) for at least 3 hrs. Wash in diH 2 O
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