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文献和实验从Cephalosporium aphidicola的培养液中分离出来的双萜。是真核细胞DNA合成的特异的可逆的抑制剂,对RNA合成及蛋白合成无抑制作用。在三种真核细胞DNA多聚酶α(复制酶)、β(修复酶)和γ(线粒体DNA复制酶)中,只抑制α。分子量为338,难溶于水,可溶于二甲亚砜和乙醇。
Synchronization, Transformation, and Cryopreservation of Suspension-Cultured Cells
levels of synchronization, either by starving the cell cultures of sucrose or by applying the toxin aphidicolin. Cell-cycle activity during cell-cycle reentry (starvation-induced synchrony) or further cell-cycle progression (aphidicolin-induced synchrony
In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts
/excision from the repair synthesis step overcome this drawback: (1) the assay is performed with purified proteins involved only at the incision step (6 ), or (2) the repair synthesis step is blocked by addition of aphidicolin (a DNA polymerase inhibitor
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