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文献和实验Medications that have a narrow therapeutic window must be regularly monitored to assist in clinical dosing. Methotrexate (MTX) is one such chemotherapeutic agent that is closely monitored using various standard assays. Patients who have renal
Generation of High-Recombinant-Protein-Producing Chinese Hamster Ovary (CHO) Cells
the dihydrofolate reductase/methotrexate system is also described.
Partial proteolysis with Staph. aureus V8 protease
mm (which is the size of the 28-tooth comb wells). Rehydrate the gel pieces in 100 microliters of rehydration/overlay buffer for 1-2 min. Do not over-hydrate. The pieces will become too fragile and will fragment. Place the gel pieces
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