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文献和实验Filter Binding Assay for EBNA-1
apparatus and incubate at filter binding temperature (4C) for 10min. Pass through filter as above and collect in a labeled tube.Load 400ul Wash Buffer into the syringe. Reattach the plunger and wash the filter immediately, collecting fraction in a separate
Fluorochrome Absorption and Emission
, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. It has a 650-nm wavelength absorption maximum (Fig. 2) and a 660-nm fluorescence emission maximum (Fig. 2). Using a 660 ?10 nm BP filter will give optimum detection for this fluorochrome
, emission monochromator, and detector. There are several crucial issues associated with both excitation and emission correction including the requirement to account for spectral band-pass and resolution, optical band-pass or neutral density filters
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