- ￥798 - 7615
- Invent
- 美国
- NE-013
- 2026年01月25日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Minute TM Nuclaer Envelope Protein Extraction Kit
- 规格：
50 Preps/4 Preps
| 规格： | 50 Preps | 产品价格： | ￥7615.0 |
|---|---|---|---|
| 规格： | 4 Preps | 产品价格： | ￥798.0 |
MinuteTM 核膜蛋白提取试剂盒
目录号：NE-013
描述
核膜蛋白是一种非常复杂的膜蛋白，由于与细胞核细胞质相连所以非常难以分离和纯化。传统方法提取和纯化需要非常大的样本量，并且过程乏味冗长。Minute核膜蛋白提取试剂盒是第一个可以快速分离天然核膜蛋白的商业试剂盒，无需使用梯度离心，超速离心。专利离心管柱技术简单，快速方便操作。起始样品量仅需10-20million个细胞，缓冲液不含表面活性剂和EDTA，操作时间<45分钟，并可获得10-50ug蛋白/样品。
应用
提取的蛋白质样品可以应用于SDS-PAGE，immunoblottings，ELISA，IP，蛋白质运输分析，酶活性检测和其他应用。
Protocol
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产品特点
简单，快速，性价比高
真正未变性的膜蛋白-无表面活性剂及EDTA
小样品量
参考文献
1. Frias A. et al. (2016). A Switch in Akt Isoforms Is Required for Notch-Induced Snail1 Expression and Protection from Cell Death. Mol Cell Biol. 2016 Mar 15; 36(6): 913–922. Prepublished online 2015 Dec 28. Published online 2016 Mar 1. doi: 10.1128/MCB.00842-15
2. Selby, T. L., Biel, N., Varn, M., Patel, S., Patel, A., Hilding, L., ... & Lowrey, A. J. (2019). The Epstein-Barr Virus Oncoprotein, LMP1, Regulates the Function of SENP2, a SUMO-protease. Scientific Reports, 9(1), 9523.doi.org/10.1038/s41598-019-45825-5
3.Shao, Y., Li, L., Liu, L., Yang, Y., Huang, J., Ji, D., ... & Sun, B. (2022). CD44/ERM/F‐actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis. Journal of Cellular and Molecular Medicine.
4.Zervopoulos, S. D., Boukouris, A. E., Saleme, B., Haromy, A., Tejay, S., Sutendra, G., & Michelakis, E. D. (2022). MFN2-driven mitochondria-to-nucleus tethering allows a non-canonical nuclear entry pathway of the mitochondrial pyruvate dehydrogenase complex. Molecular Cell, 82(5), 1066-1077
5.Kovacs, M. T., Vallette, M., Wiertsema, P., Dingli, F., Loew, D., de Freitas Nader, G. P., ... & Ceccaldi, R. (2023). DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C. Molecular Cell.
6.Zhou, X. Y., Lin, B., Chen, W., Cao, R. Q., Guo, Y., Said, A., ... & Zhu, Y. M. (2023). The brain protection of MLKL inhibitor necrosulfonamide against focal ischemia/reperfusion injury associating with blocking the nucleus and nuclear envelope translocation of MLKL and RIP3K. Frontiers in Pharmacology, 14.
7.Taga, K., & Takeuchi, H. (2024). Novel role of host protein SLC25A42 in the HIV‐1 reactivation of latent HIV‐1 provirus. Microbiology and Immunology.
8.Brajendra K. Tripathi; Nicole Hirsh; Xiaolan Qian; Marian E. Durkin; Dunrui Wang; et al.(2024).The pro-oncogenic noncanonical activity of a RAS•GTP:RanGAP1 complex facilitates nuclear protein export.Nature cancer.DOI 10.1038/s43018-024-00847-5
9.Penghui Huang; Xiaomei Zhang; Zhiyuan Cheng; Xu Wang; Yuchen Miao; et al.(2023).The nuclear pore Y-complex functions as a platform for transcriptional regulation of FLOWERING LOCUS C in Arabidopsis. The Plant Cell.DOI 10.1093/plcell/koad271
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- 作者
- 内容
- 询问日期
文献和实验1. Frias A. et al. (2016). A Switch in Akt Isoforms Is Required for Notch-Induced Snail1 Expression and Protection from Cell Death. Mol Cell Biol. 2016 Mar 15; 36(6): 913–922. Prepublished online 2015 Dec 28. Published online 2016 Mar 1. doi: 10.1128/MCB.00842-15
2. Selby, T. L., Biel, N., Varn, M., Patel, S., Patel, A., Hilding, L., ... & Lowrey, A. J. (2019). The Epstein-Barr Virus Oncoprotein, LMP1, Regulates the Function of SENP2, a SUMO-protease. Scientific Reports, 9(1), 9523.doi.org/10.1038/s41598-019-45825-5
3.Shao, Y., Li, L., Liu, L., Yang, Y., Huang, J., Ji, D., ... & Sun, B. (2022). CD44/ERM/F‐actin complex mediates targeted nuclear degranulation and excessive neutrophil extracellular trap formation during sepsis. Journal of Cellular and Molecular Medicine.
4.Zervopoulos, S. D., Boukouris, A. E., Saleme, B., Haromy, A., Tejay, S., Sutendra, G., & Michelakis, E. D. (2022). MFN2-driven mitochondria-to-nucleus tethering allows a non-canonical nuclear entry pathway of the mitochondrial pyruvate dehydrogenase complex. Molecular Cell, 82(5), 1066-1077
5.Kovacs, M. T., Vallette, M., Wiertsema, P., Dingli, F., Loew, D., de Freitas Nader, G. P., ... & Ceccaldi, R. (2023). DNA damage induces nuclear envelope rupture through ATR-mediated phosphorylation of lamin A/C. Molecular Cell.
6.Zhou, X. Y., Lin, B., Chen, W., Cao, R. Q., Guo, Y., Said, A., ... & Zhu, Y. M. (2023). The brain protection of MLKL inhibitor necrosulfonamide against focal ischemia/reperfusion injury associating with blocking the nucleus and nuclear envelope translocation of MLKL and RIP3K. Frontiers in Pharmacology, 14.
7.Taga, K., & Takeuchi, H. (2024). Novel role of host protein SLC25A42 in the HIV‐1 reactivation of latent HIV‐1 provirus. Microbiology and Immunology.
8.Brajendra K. Tripathi; Nicole Hirsh; Xiaolan Qian; Marian E. Durkin; Dunrui Wang; et al.(2024).The pro-oncogenic noncanonical activity of a RAS•GTP:RanGAP1 complex facilitates nuclear protein export.Nature cancer.DOI 10.1038/s43018-024-00847-5
9.Penghui Huang; Xiaomei Zhang; Zhiyuan Cheng; Xu Wang; Yuchen Miao; et al.(2023).The nuclear pore Y-complex functions as a platform for transcriptional regulation of FLOWERING LOCUS C in Arabidopsis. The Plant Cell.DOI 10.1093/plcell/koad271
10.Amita Singh; Victor Fourcassié; Karen Cristine Gonçalves dos Santos; Hocine Chelbi; Natacha Mérindol; et al(2025).Effects of GS-CA1 on nuclear envelope-associated early HIV-1 infection steps. Frontiers in Virology.DOI 10.3389/fviro.2025.1547176
Matrix 310 Matrix 3100 NGF Emax免疫测试系统 pGEM-T和pGEM-T Easy 载体系统 Powerplex 16 组织和毛发提取试剂盒 siLentGene™ U6 盒 RNA 干扰系统
）从倍增基因获得，也可以来自人工合成的基因。通过PCR获得感兴趣的DNA的方法是：首先，分离含有感兴趣的DNA片段的mRNA的总RNA，并通过逆转录聚合酶链反应（RT-PCR）将RNA逆转录成cDNA。根据公布的NCBI基因编码序列（CD）设计寡核苷酸引物，并在引物中加入指定的限制酶位点。然后，通过PCR扩增感兴趣的DNA的编码区，用凝胶提取试剂盒纯化后，将DNA片段插入克隆载体中。通过RT-PCR将RNA逆转录成cDNA通过PCR扩增感兴趣的DNA的编码区经验虽然从RT-PCR中获取DNA片段是常用
(PTEN ), and Multiple Endocrine Neoplasia types 1 and 2 (MEN1 and RET ). This chapter focuses on the methodologies specific to the WAVE� System for Mutation Detection 2100 (Transgenomic Inc., Omaha, NE, USA) and highlights the use of Navigator™ software
