- ￥6990
- Invent
- 美国
- MM-018
- 2026年01月25日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 英文名：
Minute™ Plant Microsomal Membrane Extraction Kit
- 规格：
50 Preps
说明：从植物中分离微粒体膜是实验室中常见实验，植物细胞中微粒体部分是植物研究项目的重点。微粒体中富含细胞膜，内质网，高尔基体，液泡膜和其他膜结构。传统的分离方法需要大量的样品量，通过超速离心来分离不同的膜结构，步骤复杂。MM-018试剂盒提供了一种快速，简单无需特殊仪器的分离方法，很小的样品量（200mg）即可提取。优化的缓冲液系统，通过简单的离心可分离水溶性胞浆蛋白和非水溶性微粒体，特别是细胞膜部分。操作时间<1小时，蛋白质得率可达100-200ug/样品
特点：
- 简单，快速，性价比高
- 得率高
- 小样品量
- 无需其他仪器
- 储存条件：储存于-20℃
参考文献：
1.Soohong Kim etal (2018). Endoplasmic reticulum stress‐induced accumulation of VAMP721/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis. Journal of Integrative Plant Biology. doi.org/10.1111/jipb.12728.
2.Zhili Yang et al(2020).PROTEIN PHOSPOHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice.Plant Cell.
3.Yu, F., Cao, X., Liu, G., Wang, Q., Xia, R., Zhang, X., & Xie, Q. (2020). ESCRT-I Component VPS23A Is Targeted by E3 Ubiquitin Ligase XBAT35 for Proteasome-Mediated Degradation in Modulating ABA Signaling. Molecular Plant, 13(11), 1556-1569.
4.Kato, T., Morita, R., Ootsuka, S., Wakabayashi, Y., Aoki, N., & Horibata, A. Evaluation of alleles at OsAGPS2, OsAGPL2, and OsSUT1 related to grain filling in rice in a common genetic background. Crop Science.
5.Hou, L.-Y.; Lehmann, M.;Geigenberger, P. Thioredoxin h2 and o1 Show Different Subcellular Localizations and Redox-Active
Functions, and Are Extrachloroplastic Factors Influencing Photosynthetic Performance in Fluctuating Light.Antioxidants 2021, 10, 705.
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- 作者
- 内容
- 询问日期
文献和实验1.Soohong Kim etal (2018). Endoplasmic reticulum stress‐induced accumulation of VAMP721/722 requires CALRETICULIN 1 and CALRETICULIN 2 in Arabidopsis. Journal of Integrative Plant Biology. doi.org/10.1111/jipb.12728.
2.Zhili Yang et al(2020).PROTEIN PHOSPOHATASE 95 Regulates Phosphate Homeostasis by Affecting Phosphate Transporter Trafficking in Rice.Plant Cell.
3.Yu, F., Cao, X., Liu, G., Wang, Q., Xia, R., Zhang, X., & Xie, Q. (2020). ESCRT-I Component VPS23A Is Targeted by E3 Ubiquitin Ligase XBAT35 for Proteasome-Mediated Degradation in Modulating ABA Signaling. Molecular Plant, 13(11), 1556-1569.
4.Kato, T., Morita, R., Ootsuka, S., Wakabayashi, Y., Aoki, N., & Horibata, A. Evaluation of alleles at OsAGPS2, OsAGPL2, and OsSUT1 related to grain filling in rice in a common genetic background. Crop Science.
5.Hou, L.-Y.; Lehmann, M.;Geigenberger, P. Thioredoxin h2 and o1 Show Different Subcellular Localizations and Redox-Active
Functions, and Are Extrachloroplastic Factors Influencing Photosynthetic Performance in Fluctuating Light.Antioxidants 2021, 10, 705.
6.Chang-Yi Chiu; Cheng-Da Tsai; J. Wang; Ming‐Hsuan Tsai; Satomi Kanno; et al.(2024).Phosphate Starvation-Induced CORNICHON HOMOLOG 5 as Endoplasmic Reticulum Cargo Receptor for PHT1 Transporters in Arabidopsis.bioRxiv (Cold Spring Harbor Laboratory).DOI 10.1101/2024.06.20.599911
7.Huikyong Cho; Induck Choi; Nadia Bouain; Amjad Nawaz; Luqing Zheng; et al.(2025). Phosphorus Availability Modulates Flowering Time Through Subcellular Reprogramming of bGLU25 and GRP7 in Flowering Plants.Developmental cell.DOI 10.1016/j.devcel.2025.10.005
8.Wen Chen; Haibin Lv; Mingwei Du; Xu Yang; Ya Zhang; et al.(2025). First molecular bases of cytochrome P450-mediated non-target-site resistance to pendimethalin in Solanum nigrum.Pesticide Biochemistry and Physiology.DOI 10.1016/j.pestbp.2025.106519
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ZOOM® IPGRunner™系统：简化的双向电泳（2D gel eletrophoresis）
Runner™夹盒放置在实验台，有覆盖膜窗口的一面朝上。包括2M Thiourea, 7 M urea, 0.5% ZOOM® Carrier ampholytes (Invitrogen, Cat. no. ZM0022), 2.0% CHAPS, 20 mM DTT 的再水化缓冲液（155μl）和E. coli裂解物被加入到IPG Runner™夹盒窗口。胶条的酸性（+）末端插入狭槽，胶面朝向夹盒的膜或覆盖面。用密封带（包含在试剂盒中）封闭加样孔，提供隔绝的再水化环境。胶条在室温下再水化8-16小时
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) per well. Invert gently several times during a 5 minute room temperature incubation. Let stand at room temperature for 1 minute before transferring to filter plate. 7. Using a wide bore pipette tip, transfer the lysate to a Qiagen TurboFilter™ (Qiagen
