S/P 3 Trial kits, 3.5KD 54MM,

Detection of apoptotic process in situ using immunocytochemical and TUNEL assays

during apoptosis. The tests were performed on sections of rat, chichen, frog and fish thymus. All the thymus were fixed in Bouin's fixative and embedded in paraffin wax (3). A) Immunocytochemical procedure The low molecular weight DNA fragments

一篇搞定 IP、co-IP 实验

糖，建议进行 Pre-clear；如果使用磁珠，此步可忽略 抗 X 抗体的轻链（25 kD）和重链（50 kD） 更侧重于如何在实验上进行优化（见下文） *注意：总蛋白上样量过多也会造成 非特异性结合，建议上样量为 500 ug-1 mg。上样之前一定要先用 BCA 定个量！定个量！定个量！ pre-clear：上样前先用裂解液过一遍柱子，减少基质本身对蛋白的吸附 Output：在某些 co-IP 实验中，实验人员会把IP后的上清分别进行诱饵蛋白 X 和靶蛋白 Y 的 WB 检测

Analysis of Oligosaccharide Ligands by High Perfor

or Tris buffer with pH>7). A relatively lower linear velocity (~0.04 cm/sec or 0.3 ml/min for 250 mm x 4.6 mm i.d. column) is highly recommended 2. The KD value can be calculated according to the simplified equation