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文献和实验Antibody Staining of Imaginal Discs
Antibody Staining of Imaginal Discs From DIS- Thom Kaufman's lab 1. Dissect tissues in Tri-PBS, gather in a 1.5 ml tube of Tri-PBS on ice(at least 10 animals can be processed in one tube). Tear larvae
, the discs were washed for 10 minutes each with the following buffers: 50% formamide, 2xSSC (30mM sodium citrate dihydrate, 0.3M sodium chlorine), 0.1% Triton X-100, 0.3% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate) (wash buffer
BAC DNA Purification For Injection
buffer (200 mM NaOH, 1% SDS); invert slowly 4-6 times; incubate 5 min at RT Add 50 ml pre-chilled P3 buffer; invert slowly 4-6 times; incubate on ice 30 min Spin 60 min at 15000xg at 4°C; filter supernatant through Whatman filter paper
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