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文献和实验3.75ml 4M NaCl 3ml 10% Triton X (Tx-100) 300µl 10% NAD 21.45ml H20 ChIP wash buffer 30ml final volume: 150µl 2M Tris 1.7ml 4.4M LiCl 60µl 0.5M EDTA 1.5ml 10% NAD 1.5ml 10% NP-40 25.09ml
real time sybergreen concentration-Real-Time PCR
to titrate in the amount your reactions need. -tfitzwater- It is written on a SG I box that 1:1000 of 10000X (thus 10X) give 0.56 optical density at 494 nm. It should be about 10^-5M. Thus X is about 10^-6 M. -Mykhaylo- QUOTE
analysis of genomic DNA by southern blotting
, agarose gel electrophoresis, gel extraction of appropriate band. Resuspend in 10 mM Tris. Label 25-50 ng cDNA-probe with 32 P using the Prime It® II Random Primer Labeling Kit (Stratagene #300385). Supplies & Equipment:
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