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文献和实验Large Scale Tubulin Preparation
and equilibrated for use. Solutions & Supplies : 220 grams Phosphocellulose 5L 0.5N NaOH 5L 0.5N HCl 13L 0.5M K-Phosphate, pH 6.8 5L ddH2 0 5 2L beakers 12L CB + 1M KCl 20L CB 300 ml of 30 mg/ml BSA in CB
Large Scale Tubulin Preparation
0.5N HCl 13L 0.5M K-Phosphate, pH 6.8 5L ddH2 0 5 2L beakers 12L CB + 1M KCl 20L CB 300 ml of 30 mg/ml BSA in CB (filtered) 1-1.5L cleaned column housing 2 stirring rods 2 aspirators with large traps
well, packed, treated with BSA to block irreversible binding sites and equilibrated for use. Solutions & Supplies: 220 grams Phosphocellulose 5L 0.5N NaOH 5L 0.5N HCl 13L 0.5M K-Phosphate, pH 6.8 5L ddH20 5 2L
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