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文献和实验Southern Hybridization Experiment Kit
This kit contains materials for six groups to perform Southern transfer and hybridization analysis using the included lambda DNA samples and biotinylated probe. The intellectual objective of the experiment is to determine the region
column into a clean 1.5 mL micro centrifuge tube. 10) Add 15-20 ul Elution Buffer(10mM Tris, pH 8.5) or water to the center of the membrane, incubate at room temperature for 3-5 minute. 11) Centrifuge at maximal speed ($13,000 x g
temperature. Discard the flow-through. 10) Repeat Steps 8–9 once. 11) Centrifuge the spin cartridge at 12,000 × g for 1–2 minutes to dry the membrane with bound RNA. Discard the collection tube and insert the spin cartridge into a recovery
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