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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
in deionized water. 12. Cover the sections with 2% aqueous solution of BSA for 10 min at room temperature. 13. Wash in deionized water. 14. Immerse in PBS for 5 min. 15. Cover the sections with Extra-avidin Peroxidase diluited 1:10-1:20 in water
3.75ml 4M NaCl 3ml 10% Triton X (Tx-100) 300µl 10% NAD 21.45ml H20 ChIP wash buffer 30ml final volume: 150µl 2M Tris 1.7ml 4.4M LiCl 60µl 0.5M EDTA 1.5ml 10% NAD 1.5ml 10% NP-40 25.09ml
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
-treatment conditions.Histochem . J. 18 :271. Rodriguez, J., and F. Deinhardt. 1960. Preparation of a semipermanent mounting medium for fluorescent antibody studies. Virology 12 :316. Rosenberg, W.S., X.O. Breakefield, C. DeAntonio, and O
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