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文献和实验Chromatin Immunoprecipitation (ChIP)
-deficient cells. Surprisingly, ~10% of the candidate sites that exhibited strong signals in the original ChIP-chip analysis, as well as in follow-up ChIP experiments performed with individual PCR primer pairs, yielded positive results of similar
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
dilution). Multiple ChIP reactions can be performed from one 3C ligation reaction. The same ligation reaction can be split and used for ChIP analyses with different antibodies. 24. Add 4-10 µg of the antibody of choice
.D.600 equivalents). Add appropriate volume of primary antibody. Incubate overnight 4°C on Nutator. I usually set up duplicate IPs. Note: Always do NO Ab control. Also, if adding competitor antigen, do so several minutes before adding Ab
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