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文献和实验For an in-depth review of the method, see Friedman, K. and B. Brewer (1995) Analysis of replication intermediates by two-dimensional agarose gel electrophoresis. Meth. Enzymol. 262: 613-627. Two dimensional agarose gels have proven
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
. It has been tested with antibodies against cyclin D1 (Pharmingen cat. no. 14561A, clone G12-4326), cyclin D2/D3 (cat. no. 14711A, clone G107-22) and cyclin E Procedure Prepare the cell type of interest as a single cell suspension and wash once with cold PBS
Protein Kinase C as a Modulator of 5- HT1A and Dopamine- D2 Receptor Signaling
Since its identification as a family of protein kinases that is activated in the presence of calcium, phosphatidyl serine, and diacylglycerol (DAG), protein kinase C (PKC) has been regarded as one of the early steps in receptor signaling
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