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文献和实验Primer Design(Manual and Automated Primer Design for SEQ and PHY Gaps)
General Primer General Primer Design Guidelines Note the 5’-3’ direction of the contig. Locate primers 100 to 200 bp from the feature. Pick primer from ≥ 2x high quality sequence coverage
Real-Time Primer Design for DNA Chips
an optimization of a specified primer set in real-time.1 IntroductionBoth, the amplification of DNA sequences using polymerase chain reaction (PCR) and the massive parallel analysis of genes in biological cells using DNA chips (or DNA arrays) have a great impact
Optimal arbitrary primer length for Differential Display(图)
is determined by statistical consideration that each primer will recognize 50-100 mRNA species. To do so, these primers have to hybridize as 6-8mers (Liang and Pardee, Science. 1992, 257:967.). In practice, however, primers shorter than 9 bases failed to be used
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