20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.01% IGEPAL CA-630, pH 7.5 @ 25°C
保存温度
-20°C
反应条件
1X First-Strand Buffer, 10 mM DTT, 200 units ABScript II Reverse Transcriptase, supplemented with 0.5 mM dNTPs (not included) and 5 uM dT23VN (not included). Incubate at 42°C for 50 minutes. If random primers are used, a 10 minute incubation at
反应缓冲液或预混液
1X First-Strand Buffer Reaction Buffer: 50 mM Tris-HCl, 75 mM KCl, 3 mM MgCl2, pH 8.3 @ 25°C