FauI内切酶报价

特别提示：包括FauI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：FauI内切酶
英文名称：FauI
产品货号：GS2158
产品规格：50U

储存条件：冷冻（-20℃）

使用说明

The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 55℃ in a total reaction volume of 50μl.
Recognition sites	CCCGC(N)4↑
来源	E.coli strain that carries the cloned Fau I gene from Flavobacterium aquatili
Assayed on	Lambda DNA
Optimal buffer	B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.)
Optimal temperature	55℃
Storage conditions	10mMTris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycero;Store at -20℃.
Ligations	After 2-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and of these 95%can be recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 4 u.a.of enzyme for 16hoursat 55℃
Methlation effect	Blocked by CG methylation.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	SmuI

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