Bsa29I内切酶北京品牌

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产品名称：Bsa29I内切酶
英文名称：Bsa29I
产品货号：GS2156
产品规格：500U

储存条件：冷冻（-20℃）

使用说明

To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dam-)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	AT↑CGAT
来源	Bacillus stearothermophilus 29
Assayed on	Lambda DNA(dam-)
Optimal buffer	G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)+ BSA
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 20-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Blocked by overlapping Dam methylation(GmATC):GATCGATC.|Blocked by CG methylation.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	BanIII,BseCI,BshVI,BsiXI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ClaI,ZhoI

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