Bpu10I内切酶现货供应

特别提示：包括Bpu10I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：Bpu10I内切酶
英文名称：Bpu10I
产品货号：GS2135
产品规格：100U

储存条件：冷冻（-20℃）

使用说明

High enzyme concentration may result in star activity or incomplete DNA cleavage.We recommend increasing incubation time instead of using an excess of Bpu10I.The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0.5.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	CC↑TNAGC
来源	E.coli strain,that carries recombinant plasmids
Assayed on	Lambda DNA
Optimal buffer	K(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;100 mM KCl;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;50%glycerol.Store at -20℃
Ligations	After 5-fold overdigestion with enzyme 80%of the DNA fragments can be ligated.Of these 90%can be recut.In the presence of 10%PEG ligation is better.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 1 hours at 37℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.

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