ALL-INCLUSIVE 3D RAT BLOOD BR

 ALL-INCLUSIVE 3D RAT BLOOD BRAIN BARRIER SPHEROID FORMATION KIT描述：
         The blood brain barrier (BBB) is a specialized capillary bed that separates the brain from the circulatory system and protects the brain from most pathogens.  Endothelial tight junctions supported by pericytes and astrocytes are primarily responsible for the highly selective nature of the BBB, restricting the passage of numerous solutes, most antibodies, and some antibiotics.  As such, efforts to understand the mechanisms underlying BBB integrity have been critical to developing techniques that are able to penetrate the BBB to deliver therapeutic or diagnostic molecules to the brain.  Due to the complexities of the BBB, it is difficult to study in a 2-dimensional  in vitro system, which inherently lacks multiple aspects of the physiological microenvironment.  In ScienCell™’s 3-dimensional rat blood brain barrier spheroid model, rat brain microvascular endothelial cells, pericytes, and astrocytes are co-cultured in low adhesion conditions, and self-assembled into 3D spheroids. Within spheroids, cells are able to interact with one another to recapitulate the key aspects of BBB, including the expression of ZO1 tight junction marker on the surface of the spheroids.
    血脑屏障(BBB)是一种特殊的毛细血管床，它将大脑与循环系统分开，保护大脑免受大多数病原体的侵害。周细胞和星形胶质细胞支持的内皮细胞紧密连接是血脑屏障高选择性的主要原因，它限制了许多溶质、大多数抗体和一些抗生素的通过。因此，了解血脑屏障完整性的机制对于开发能够穿透血脑屏障向大脑传递治疗或诊断分子的技术至关重要。由于BBB的复杂性，很难在二维体外系统中进行研究，因为二维体外系统本来就缺乏生理微环境等多个方面。ScienCell™的三维大鼠血脑屏障球体模型中，大鼠大脑微血管内皮细胞、周细胞和星形胶质细胞在低黏附条件下共培养，并自组装成三维球体。在椭球体内，细胞可以相互作用来概括BBB的关键方面，包括在椭球表面的ZO1紧密连接标记的表达。

Catalog No.	3D-R8738
Country of Manufacture	United States
Product Code	3D-RBBBSF
Size/Quantity
Product Use	3D-RBBBSF is for research use only. It is not approved for human or animal use, or application in clinical or in vitrodiagnostic procedures.
Storage
Shipping Info	R1000, R1200, R1800, 3D-8752, 0010-cs, 0583, 8752, 0025-cs, and 0503 are shipped on dry ice. 3D-8701, 8701, and [0343 (or) 0353 (or) 0383] are shipped at room temperature.