Ready-to-use 3D Human Cortical

Ready-to-use 3D Human Cortical Spheroids：3D人体模型-中枢神经系统

       Damage to the central nervous system (CNS) has a life-threatening impact on humans, and there is a high demand for relevant models to study CNS diseases and therapeutic strategies. Mature neurons and astrocytes in the brain together form a complex three-dimensional network system. Astrocytes  in vivo regulate blood flow, provide energy to neurons, and supply the building blocks of neurotransmitters, which support the formation of functional synapses. The complex cellular arrangement of the central nervous system are unlikely to be recapitulated in 2D cultures where cells display the planar morphology and have cellular interactions only in the lateral direction. To create a more biologically relevant  in vitro brain model, ScienCell has developed ready-to-use 3D human cortical spheroids (SP3D-HCS) comprised of primary human neurons and astrocytes. SP3D-HCS are generated by coculturing neurons and astrocytes at a 1:7 ratio in 3D culture. Immunofluorescence analysis revealed that the 3D human cortical spheroids maintain direct cell-cell interactions between neurons and astrocytes and form functional synapses throughout the spheroids. Importantly, in the defined, serum-free spheroid medium, the neurons are networked with quiescent astrocytes, a phenotype closely resembling  in vivo astrocytes. The quiescent astrocytes are critical for synapse function and neural development. This advanced and ready-to-use cortical spheroid is highly suitable for the study of CNS functions, diseases, and therapeutics.
中枢神经系统(CNS)的损伤对人类有威胁生命的影响，对研究CNS疾病和治疗策略的相关模型有很高的需求。成熟的神经元和脑内的星形胶质细胞共同构成一个复杂的三维网络系统。星形胶质细胞在体内调节血液流动，为神经元提供能量，并为神经递质提供基础材料，支持功能突触的形成。复杂的中枢神经系统的细胞排列不太可能在二维培养中被再现，因为在二维培养中细胞表现为平面形态，细胞间的相互作用只在侧面进行。为了创建一个更具有生物学相关性的体外大脑模型，ScienCell开发了随时可用的3D人类皮质球体(sp3d - hc)，由初级人类神经元和星形胶质细胞组成。sp3d - hc是由神经元和星形胶质细胞以1:7的比例混合培养而成。免疫荧光分析显示，三维人皮质球状体在神经元与星形胶质细胞之间维持直接的细胞-细胞相互作用，并在整个球状体中形成功能突触。重要的是，在明确的、无血清的椭球体培养基中，神经元与静止星形胶质细胞联网，这种表型与体内星形胶质细胞非常相似。静止的星形胶质细胞对突触功能和神经发育至关重要。这种先进的、随时可用的皮质球体非常适合研究中枢神经系统功能、疾病和治疗学。