- $1580
- ScienCell
- SP3D-5000
- 美国
- 2025年12月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
SP3D-HSteECS
- CAS号：
见产品
- 保质期：
大量
- 供应商：
中乔新舟
- 保存条件：
-20
Ready-to-use 3D Hepatic Stellate-Endothelial Cell Spheroids描述：
Activation of the quiescent hepatic stellate cell (HSteC) to a phenotype characterized by increased proliferation, migration, and synthesis of extracellular matrix is considered the pivotal event leading to fibrosis [1]. In addition to HSteC activation, another change that precedes fibrosis is capillarization of the hepatic sinusoidal endothelial cells (HSEC) [2]. In physiological conditions, HSEC maintain hepatic stellate cell quiescence, thereby inhibiting intrahepatic vasoconstriction and fibrosis development [2]. When capillarized, however, hepatic sinusoidal endothelial cells lose their capacity to inactivate hepatic stellate cells, thus promoting fibrogenesis and intrahepatic vasoconstriction [2]. Liver fibrogenesis and angiogenesis, therefore, are closely linked. For example, liver fibrosis enhances angiogenesis, and in turn, liver angiogenesis aggravates liver fibrosis. To investigate the signaling crosstalk between these cellular events, ScienCell has developed ready-to-use 3D hepatic stellate-endothelial cell spheroids (SP3D-HSteECS) comprised of hepatic stellate cells and sinusoidal endothelial cells at a 1:4 ratio. These spheroids are ready for experiments at 24 hours after thawing. Furthermore, immunostaining of the co-culture spheroids reveals the presence of vimentin-positive stellate cells and von willebrand factor (VWF)-positive sinusoidal endothelial cells. Furthermore, the spheroids also contain the activated stellate cell population marked by the smooth muscle actin (SMA) staining.
静止的肝星状细胞(HSteC)被激活形成以增殖、迁移和细胞外基质合成增加为特征的表型，这被认为是导致肝纤维化的关键事件。除了活化HSteC外，肝窦内皮细胞(HSEC)[2]的毛细血管化是纤维化前的另一个变化。在生理条件下，HSEC维持肝星状细胞静止，从而抑制肝内血管收缩和纤维化发展。然而，当毛细血管化时，肝窦内皮细胞失去了灭活肝星状细胞的能力，从而促进纤维形成和肝内血管收缩。因此，肝纤维化与血管生成密切相关。例如，肝纤维化促进血管生成，反过来，肝血管生成加重肝纤维化。为了研究这些细胞事件之间的信号串扰，ScienCell开发了随时可用的3D肝星状内皮细胞球体(SP3D-HSteECS)，由肝星状细胞和窦状内皮细胞以1:4的比例组成。这些球状体在解冻后24小时就可以进行实验了。此外，对共培养的球状体进行免疫染色，发现vimentin阳性的星状细胞和VWF阳性的窦状内皮细胞。此外，这些球状体还含有被平滑肌肌动蛋白(SMA)染色的活化的星状细胞群。
| Catalog No. | SP3D-5000 |
|---|---|
| Country of Manufacture | United States |
| Product Code | SP3D-HSteECS |
| Size/Quantity | |
| Product Use | SP3D-HSteECS are for research use only. It is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures. |
| Storage | |
| Shipping Info | SP-5000, 3D-5452, 0583 are shipped on dry ice. 3D-5201, and (0343 or 0353 or 0383) are shipped at room temperature. |
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Isolation and Culture of Hepatic Stellate Cells
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