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- Adipogen
- AG-CN2-0013-C250
- 瑞士
- 2025年07月16日
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99
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欣博盛生物
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250 ug
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文献和实验-CN2-0013-C250 alpha-Galactosylceramide [alpha-Gal-Cer; KRN7000] α-半乳糖神经酰胺，NK 细胞激活剂 250 ug BML-SL232-0100 KRN7000 α-半乳糖神经酰胺 100 ug
Replication timing by density transfer
), arrest with 200 nM alpha factor (1-1.25 doubling times, until % unbudded cells stops increasing). NOTE: This alpha factor concentration is for bar1 strains. BAR1+ strains require 15x more alpha factor. 3. Filter, wash in Ycomp+alpha factor, resuspend
Replication timing by comparative hybridization
cells per time point. (Raffinose has not worked well for me, so I avoid it.) 2. Arrest the culture with alpha factor (200 nM final concentration for bar1 strains) when the OD660 is about 0.2-0.25. 3. When the cells have arrested (more than 95
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