AfeI内切酶北京厂家

特别提示：包括AfeI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：AfeI内切酶
英文名称：AfeI
产品货号：GS2160
产品规格：100U

储存条件：冷冻（-20℃）

使用说明

The minimum number of units that resulted in complete digestion of 1μgof substrate DNA in 16hoursis 0,25.AfeI cleaves supercoiled and linear plasmid DNA(pBR322)at a roughly equal rate.AfeI cleaves Lambda DNA/BamHI digest at a rate 3-4 times higher than plasmid DNA.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(BamHI-digest)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	AGC↑GCT
来源	E.coli strain that carries the cloned Afe I gene from Alcaligenes faecalis T2774
Assayed on	Lambda DNA(BamHI-digest)
Optimal buffer	Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10 mM Tris-HCl(pH 7.6);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme more than 80%of DNA pBR322 fragments can be ligated and recut.
Non-specific hydrolisis	fragments can be ligated and recut.|Non-specific hydrolisis No nonspecific activity was detected after incubation of 1μgof DNA with 40 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	Aor51HI,Eco47III,FunI

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