Ple19I内切酶现货供应

特别提示：包括Ple19I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：Ple19I内切酶
英文名称：Ple19I
产品货号：GS2152
产品规格：100U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(HindIII-digest)in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	CGAT↑CG
来源	Pseudomonas lemoignei 19
Assayed on	Lambda DNA(HindIII-digest)
Optimal buffer	Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	37℃
Storage conditions	10 mM Tris-HCl(pH 7.5);100 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 3-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Not blocked by overlapping Dam-methylation(GmATC):CGATCG
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	BpvUI,BspCI,MvrI,PvuI

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