Tru9I内切酶优惠促销

特别提示：包括Tru9I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：Tru9I内切酶
英文名称：Tru9I
产品货号：GS2154
产品规格：300U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl.
Recognition sites	T↑TAA
来源	E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9
Assayed on	Lambda DNA
Optimal buffer	W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)
Optimal temperature	65℃
Storage conditions	10mMTris-HCl-(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol Store at -20℃.
Ligations	After 20-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 40 u.a.of enzyme for 16hoursat 65℃.
Methlation effect	Blocked by TTAmA methylation.
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	MseI,SaqAI,Tru1I

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