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文献和实验Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes
integral membrane proteins that share significant sequence similarity. Of the established glucose transporter isoforms, GLUT-4 is highly expressed in adipose tissue and striated muscle (3 ). In the basal state, GLUT-4 cycles slowly between the plasma
Basic Protocol 1: Standard Native Gels for High‐Resolution Separation of Protein Complexes Materials Separating and sample gel solutions (Table 19.18.1 ) Samples of soluble proteins or solubilized
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
interaction, the LexA‐VP16 transcription factor is immobilized at the membrane and is unable to reach the nucleus of the yeast cell. Consequently, the reporter genes integrated into the yeast genome are silent. (B ) Using a soluble protein as bait. Soluble
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