Adipose Protein Fractionation

Fractionation Analysis of the Subcellular Distribution of GLUT-4 in 3T3-L1 Adipocytes

integral membrane proteins that share significant sequence similarity. Of the established glucose transporter isoforms, GLUT-4 is highly expressed in adipose tissue and striated muscle (3 ). In the basal state, GLUT-4 cycles slowly between the plasma

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

Basic Protocol 1: Standard Native Gels for High‐Resolution Separation of Protein Complexes   Materials Separating and sample gel solutions (Table 19.18.1 ) Samples of soluble proteins or solubilized

Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions

interaction, the LexA‐VP16 transcription factor is immobilized at the membrane and is unable to reach the nucleus of the yeast cell. Consequently, the reporter genes integrated into the yeast genome are silent. (B ) Using a soluble protein as bait. Soluble