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- 文献和实验
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- 供应商:
上海觅拓
- 规格:
100 assays
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文献和实验Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
5 seconds again. Quick spin 30 seconds at maxi speed at RT. 6: Transfer cytoplasmic fraction to a new tube, put nuclear samples on ice now. 7: T℃ytoplasmic fractions, add 0.9ml 1.2xTRIzol reagent. Vortex to mix completely, sit at RT for 5 minutes
Oocyte isolation, maturation, enucleation and fractionation
; pH 7.4) -- take care not to lyze or damage embryos. (Wash embryos 3X if you are using RNAlater). Add 1 ml of TRIzol reagent (located in the 4æC fridge next to Chi’s bench). Homogenize sample by pipetting up and down so sample
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RNA
at RT.6: Transfer cytoplasmic fraction to a new tube, put nuclear samples on ice now.7: T℃ytoplasmic fractions, add 0.9ml 1.2xTRIzol reagent. Vortex to mix completely, sit at RT for 5 minutes.8: Add 200μl chloroform:IAA 200μl per 1ml TRIzol. Vortex
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