- 询价
- Cell Signaling Technology
- 3873
- USA
- 2025年10月01日
- western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）
- 小鼠
- 人,小鼠,大鼠,驴
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
α-Tubulin (DM1A) Mouse mAb
- 抗原：
/
- 应用范围：
western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）
- 宿主：
小鼠
- 适应物种：
人,小鼠,大鼠,驴
- 库存：
大量
- 抗原来源：
/
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
α-Tubulin (DM1A) Mouse mAb #3873
PhosphoSitePlus ® protein, site, and accession data: TUBA1B
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R Mk | Endogenous | 52 | Mouse IgG1 |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
α-Tubulin (DM1A) Mouse mAb detects endogenous levels of total α-tubulin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length chicken α-tubulin purified from brain extracts, and recognizes residues surrounding Val440 of α-tubulin protein.
Western Blotting
Western blot analysis of extracts from HeLa and COS-7 cells using α-Tubulin (DM1A) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using α-Tubulin (DM1A) Mouse mAb.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using α-Tubulin (DM1A) Mouse mAb (green). Red = Propidium Iodide (fluorescent DNA dye).
Background
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
Application References
- Hebel, K. et al. (2011) J Immunol 187, 5627-35. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2128 β-Tubulin (9F3) Rabbit mAb
- 2144 α-Tubulin Antibody
- 2146 β-Tubulin Antibody
- 2148 α/β-Tubulin Antibody
- 7072 Phototope® -HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 8112 SignalStain® Antibody Diluent
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
For Research Use Only. Not For Use In Diagnostic Procedures.
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- 作者
- 内容
- 询问日期
文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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