- 询价
- Cell Signaling Technology
- 3350
- USA
- 2025年11月22日
- W, IP
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
ApoA1 (5F4) Mouse mAb
- 抗原：
full-length recombinant human ApoA1
- 应用范围：
W, IP
- 供应商：
CST
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 适应物种：
H
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 25 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | ApoA1 (5F4) Mouse mAb detects endogenous level of total ApoA1 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a full-length recombinant human ApoA1. Western Blotting
Western blot analysis of decreasing concentrations of ApoA1 protein purified from human serum using ApoA1 (5F4) Mouse mAb. Western Blotting
Western blot analysis of extracts from HepG2 cells using ApoA1 (5F4) Mouse mAb. |
| Background | ApoAI (Apolipoprotein A1) is a major component of high density lipoprotein (HDL, the ?good cholesterol?) in plasma. It is produced in the liver and small intestine. ApoA1 is a cofactor for lecithin cholesterolacyltransferase (LCAT) that is responsible for the formation of plasma cholesteryl esters and promotes cholesterol efflux from tissues to the liver for excretion. Defects in ApoA1 are associated with high density lipoprotein deficiency (HDLD) and systemic non-neuropathic amyloidosis (1-3). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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