- 询价
- Cell Signaling Technology
- 5179
- USA
- 2026年05月28日
- 免疫荧光（IF）
- 兔
- 人,驴,细菌,狗,马
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- 文献和实验
- 技术资料
- 抗体英文名：
Sox2 (D6D9) XP Rabbit mAb (Alexa Fluor 555 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）
- 宿主：
兔
- 级别：
详见MSDS文件
- 抗原来源：
/
- 保质期：
详见说明书
- 适应物种：
人,驴,细菌,狗,马
- 库存：
大量
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Wnt / Hedgehog / Notch
Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #5179
PhosphoSitePlus ® protein, site, and accession data: SOX2
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC | H (Mk) (B) (Dg) (Hr) | Endogenous | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human Mk=Monkey B=Bovine Dg=Dog Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5179:
- Immunofluorescence
Specificity / Sensitivity
Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) detects endogenous levels of Sox2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2.
IF-IC
Confocal immunofluorescent analysis of NTERA2 (left) and HeLa cells (right) using Sox2 (D6D9) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) (red). Actin filaments were labeled with Alexa Fluor® 647 phalloidin (blue).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 555 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Sox2 (D6D9) XP® Rabbit mAb #3579.
Background
Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).
- Nichols, J. et al. (1998) Cell 95, 379-391.
- Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
- Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
- Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
- Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
- Boyer, L.A. et al. (2005) Cell 122, 947-956.
- Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.
- Bass, A.J. et al. (2009) Nat Genet 41, 1238-42.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验性生物素所致的假阳性染色，其检测过程省去一个步骤为检测过程节省了时间，并且其与所有基于过氧化物酶的底物相容。抗体：Sox2 (D6D9) XP. Rabbit mAb #3579样本：石蜡包埋的人类肺部肿瘤检测：基于生物素的检测（左）或基于聚合物的检测（SignalStain. Boost IHC Detection Reagent #8114;（右）色原二氨基联苯胺 (DAB) 底物是基于过氧化物酶检测系统中最常用的色原之一。DAB 与 HRP 反应以在抗体结合处形成棕色沉淀物。在实验中建议始终
稀释液的效果更好。4. 匹配的二抗和显色系统——「配一配和滴一滴」使用多聚 HRP（Poly-HRP）的二抗，即在二抗上连接大量 HRP，以充分放大信号；同时，配合优质的色原产品，提升灵敏度，增强信号。注：在人肺癌标本中使用 Sox2(D6D9) XP® RabbitmAb#3579 检测 Sox2 的表达，使用基于多聚-HRP 的 SignalStain® BoostIHCDetectionReagent 的检测灵敏度更高。注：DAB 色原比较：在人乳腺癌组织中使用 p-Stat3(Tyr705)(D
Recombinant Rabbit mAb (Alexa Fluor® 647 conjugate) (S-712-10) 二、抗独特型抗体（Anti-idiotype antibody） 抗独特型抗体更适合单一CAR 骨架的深度表征，它识别scFv可变区的专属独特表位，具有极强的靶点特异性，仅识别特定scFv序列构建的CAR，几乎不存在交叉反应。目前市面上已有多款成熟商业化抗独特型抗体，分别识别临床广泛使用的CAR scFv克隆，包括识别FMC63 scFv（CD19 CAR-T）、人源2C6 scFv
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