- 询价
- Cell Signaling Technology
- 4218
- USA
- 2025年12月09日
- W, IP
- H,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
LSD1 (1E5-H2) Mouse mAb
- 抗原：
recombinant protein fragment corresponding to the human LSD1 protein
- 应用范围：
W, IP
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
H,Mk
- 供应商：
CST
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H Mk | Endogenous | 110 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | LSD1 (1E5-H2) Mouse mAb detects endogenous levels of total LSD1 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment corresponding to the human LSD1 protein. Western Blotting
Western blot analysis of extracts from various cell types using LSD1 (1E-5H2) Mouse mAb. |
| Background | Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor (1). Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes (2,3). In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1 (4,5). LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells (1,6,7). In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation (8). Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine (8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验pellets in 10 ml of 100 mM Tris buffer or Binding buffer. And pool into 50 ml Falcon tubes (try not to make subbles).Use 3.2 ml/cm 12-14,000 MW dialysis tubing. Heat tubing in 500 ml H2O in microwave. Not boiling. Rinse tubing in fresh H2O several times
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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