- 询价
- Cell Signaling Technology
- 8884
- USA
- 2026年05月29日
- W
- H,M,R,Mk
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- 文献和实验
- 技术资料
- 抗体英文名：
GAPDH (D16H11) XP Rabbit mAb (HRP Conjugate)__®
- 抗原：
synthetic peptide corresponding to residues near the carboxy terminus of human GAPDH protein
- 应用范围：
W
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
H,M,R,Mk
- 级别：
详见MSDS文件
- 库存：
大量
- 标记物：
HRP
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 37 | Rabbit |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate) detects endogenous levels of total GAPDH protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GAPDH protein. Western Blotting
Western blot analysis of extracts from NIH/3T3 and HeLa cells using GAPDH (D16H11) XP® Rabbit mAb (HRP Conjugate). |
| Description | This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated GAPDH (D16H11) XP® Rabbit mAb #5174. |
| Background | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
性生物素所致的假阳性染色，其检测过程省去一个步骤为检测过程节省了时间，并且其与所有基于过氧化物酶的底物相容。抗体：Sox2 (D6D9) XP. Rabbit mAb #3579样本：石蜡包埋的人类肺部肿瘤检测：基于生物素的检测（左）或基于聚合物的检测（SignalStain. Boost IHC Detection Reagent #8114;（右）色原二氨基联苯胺 (DAB) 底物是基于过氧化物酶检测系统中最常用的色原之一。DAB 与 HRP 反应以在抗体结合处形成棕色沉淀物。在实验中建议始终
我的石蜡切片又「团灭」了！没关系，专业技术人员与您并肩推塔！
Antibody Diluent #8112 稀释PD-L2抗体（#82723）稀释度为1：200。11. 横沥封闭液，用卫生纸尽量吸干封闭液后，将一抗滴加在切片上。（此步建议一张张完成，防止干片）12. 将湿盒小心移入4度冰箱，过夜。CST技术人员正在配置和滴加抗体二抗孵育与显色13. 小心取出湿盒，在室温平衡45分钟左右。14. 洗涤：切片浸没于TBST中，3 ×5 min。15. 将二抗SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)#8114
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