Phospho-NF-κB p65 (Ser536) (93

Product Pathways - NF-kB Signaling

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Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IF-IC F	H M R Hm Mk Pg (Dg)	Endogenous	65	Rabbit IgG

Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

3033:

Flow , Immunofluorescence , Immunoprecipitation , Western Blotting

Specificity / Sensitivity

Phospho-NF-kappaB p65 (Ser536) (93H1) Rabbit mAb detects NF-κB p65 only when phosphorylated at Ser536. It does not cross-react with the p50 subunit or other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-κB p65.

Background

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

Application References

• Lacasa, D. et al. (2007) Endocrinology 148, 868-77. Applications: Western Blotting
• Suzuki, S. et al. (2007) J Biol Chem 282, 25177-81. Applications: Western Blotting
• Lou, H. and Kaplowitz, N. (2007) J Biol Chem 282, 29470-81. Applications: Western Blotting
• Peiser, M. et al. (2008) J Leukoc Biol 83, 1118-27. Applications: ELISA
• Luedde, T. et al. (2008) Proc Natl Acad Sci U S A 105, 9733-8. Applications: Western Blotting
• Vince, J.E. et al. (2008) J Cell Biol 182, 171-84. Applications: Western Blotting
• Yadav, U.C. et al. (2009) Invest Ophthalmol Vis Sci 50, 2276-82. Applications: IF-P (paraffin)
• Dai, P. et al. (2009) J Immunol 182, 3450-60. Applications: Western Blotting
• Milsom, M.D. et al. (2009) Blood 113, 5111-20. Applications: Western Blotting
• Liu, M. et al. (2009) Am J Pathol 174, 1910-20. Applications: Western Blotting
• Yamanaka, Y. et al. (2009) Blood 114, 3265-75. Applications: Western Blotting
• Solt, L.A. et al. (2009) J Biol Chem 284, 27596-608. Applications: Western Blotting
• Wang, H. et al. (2009) J Immunol 183, 4755-63. Applications: IHC-F (floating)
• Xie, S. et al. (2010) J Immunol 184, 2289-96. Applications: Flow Cytometry IF-IC (In Cells) Western Blotting
• Kwong, C. et al. (2011) J Immunol 186, 1781-9. Applications: Western Blotting
• Stairs, D.B. et al. (2011) Cancer Cell 19, 470-83. Applications: Western Blotting
• Dental, C. et al. (2011) J Virol , . Applications: Flow Cytometry

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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.