DJ-1 (D29E5) XP® Rabbit mAb

5933:

Immunofluorescence, Immunoprecipitation, Western Blotting

DJ-1 (D29E5) XP^® Rabbit mAb recognizes endogenous levels of total DJ-1 protein.

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein.

Western Blotting

Western blot analysis of extracts from MEF wild-type, MEF DJ-1 (-/-), HeLa, and C6 cells using DJ-1 (D29E5) XP^® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

IF-IC

Confocal immunofluorescent analysis of MEF wild-type (left) or MEF DJ-1 (-/-) (right) cells using DJ-1 (D29E5) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany).

Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).

1. Borrelli, E. (2005) Neuron 45, 479-81.
2. Bonifati, V. et al. (2003) Science 299, 256-9.
3. Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13.
4. Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63.
5. Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93.
6. Goldberg, M.S. et al. (2005) Neuron 45, 489-96.

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• 4179 α-Synuclein (D37A6) XP^® Rabbit mAb
• 4211 Parkin (Prk8) Mouse mAb
• 5559 LRRK2 Antibody
• 5660 PINK1 (N4/15.11) Mouse mAb
• 5560 DJ-1 (D21E11) Rabbit mAb
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
• 9998 BSA
• 9999 Nonfat Dry Milk

For Research Use Only. Not For Use In Diagnostic Procedures.