- 询价
- Cell Signaling Technology
- 5677
- USA
- 2025年11月13日
- ChIP
- H,M
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated)
- 抗原：
recombinant protein specific to the amino terminus of human Oct-4A protein
- 应用范围：
ChIP
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
H,M
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 immunoprecipitations)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 immunoprecipitations) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
more info application references datasheet PDF MSDS PDF protocols
Applications Key: ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| ChIP | H M | Endogenous | 45 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) detects endogenous levels of total Oct-4A protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A protein. Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NTERA-2 cells and either 10 μl of Nanog (D73G4) XP® Rabbit mAb (ChIP Formulated) #5232, Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) #5677, Sox2 (D6D9) XP® Rabbit mAb (ChIP Formulated), or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human Nanog promoter primers, SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mES cells and either 10 μl of Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Oct-4A (C30A3C1) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
| Background | Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验实际上将作为阴性对照使用。CST 推荐使用 Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) #4620。该抗体可检测与基因组中所有 DNA 序列结合的所有组蛋白 H3 类型（H3、H3.3、CENP-A）。因此，不管检测的位点活性状态如何，该抗体都能为 ChIP 实验提供通用的阳性对照。 阴性对照 阴性对照抗体（如正常兔 IgG）不能识别特异抗原表位，因此可用于检测非特异性结合。例如，如果阴性对照样品中的产物量等于特异
法所替代。利用微球菌核酸酶的切割特性（在染色质核小体之间的 DNA 更容易被切开），可以快速温和地将染色质 DNA 切断，且最大限度地保持了细胞原有染色质结构以及目的蛋白与 DNA 的结合状态，使 ChIP 的准确性和结果可信度大大提高。CST 推出的 SimpleChIP® 酶解法试剂盒，含有标准 ChIP 流程所需的整套试剂、ChIP 级蛋白质 G 微珠、阳性对照抗体（Histone H3(D2B12) XP® Rabbit mAb (ChIP Formulated) #4620）、阴性对照抗体、DNA
Immunohistochemisty-Enzymatic Protocol
, mount in OCT and freeze at -20 to -80° C. Cut 5 - 15 µm thick tissue sections using a cryostat. Thaw-mount the sections onto gel-coated slides. Refer to the Support Protocols ection to follow for instructions on how to prepare gel-coated
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