- 询价
- Cell Signaling Technology
- 9863
- USA
- 2025年11月21日
- IP
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
Protein A Agarose Beads
- 应用范围：
IP
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
1 ml (50 immunoprecipitations)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 1 ml (50 immunoprecipitations) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
Product Pathways - Complementary Reagents
Protein A Agarose Beads #9863
Protocols
- 9863:
- Immunoprecipitation
Description
Protein A Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from immunoprecipitations (IP assays). Protein A is covalently coupled to agarose beads. Protein A exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat, and sheep) and can be used for immunoprecipitation assays with these antibodies.
IP
Immunoprecipitation (IP) of COS-7 cell extracts using Ubc12 (D13D7) Rabbit mAb #5641 and Protein A Agarose Beads #9863. Western blot analysis was performed on the IP pellet (lane 1) and supernatant (lane 2) using Ubc12 (D13D7) Rabbit mAb #5641.
Directions for Use
Vortex tube briefly to resuspend the beads. Add 20-40 μl of bead slurry to each immunoprecipitation (IP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube. Please follow CST's recommended IP protocol to perform IP followed by western blot.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 9803 Cell Lysis Buffer (10X)
- 9806 RIPA Buffer (10X)
- 3423 Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose Bead Conjugate)
- 7722 Blue Loading Buffer Pack
- 7723 Red Loading Buffer Pack
For Research Use Only. Not For Use In Diagnostic Procedures.
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- 内容
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文献和实验Encapsulation of Cells in Agarose Beads
For the analysis of large DNA fragments using pulsed-field gel electrophoresis (PFGE), it is necessary to first embed the cells in agarose to prevent shearing of the DNA during protein extraction and restriction enzyme digestion. The speed
Purification of DNA Fragments from Agarose Gels Using Glass Beads
Size selection of DNA fragments is frequently required before ligation or labeling for the preparation of probes. Many methods are available for purifying DNA fragments following electrophoresis in agarose gels, including the use of low-melting
PREPARATION OF PROTEIN A SEPHAROSE CL 4B BEADS
Swelling and Storage1.Resuspend .2 gm of beads (= 1.0 ml swelled bead volume)in 40 ml of distilled water.Let swell for at least 2 hours.2.Wash beads twice in (10 ml each wash).Spin in clinical or table top fuge.Do not exceed 2K rpm.3.Resuspend
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