Phospho-p44/42 MAPK (Erk1/2) (
Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb (HRP Conjugate)__®- 询价
- Cell Signaling Technology
- 8544
- USA
- 2026年05月29日
- W
- H,M,R,Hm,Mk,Mi,Dm,Z,B,Dg,Pg,Sc,Ce
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- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb (HRP Conjugate)__®
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase
- 应用范围：
W
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
H,M,R,Hm,Mk,Mi,Dm,Z,B,Dg,Pg,Sc,Ce
- 供应商：
CST
- 标记物：
HRP
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Dg=Dog Pg=Pig Sc=S. cerevisiae Ce=C. elegans
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Hm Mk Mi Dm Z B Dg Pg Sc (Ce) | Endogenous | 44, 42 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (HRP Conjugate) detects endogenous levels of p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Thr202. This antibody does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinases. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase. Western Blotting
Western blot analysis of extracts from either Jurkat cells, treated with U0126 #9903 (10 µM, 1 hr) or TPA (200 nM, 10 min), or MCF7 cells, treated with λ phosphatase or TPA, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (HRP Conjugate). |
| Description | This Cell Signaling Technology® antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370. |
| Background | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验tctgtttcat cagaaatga // 我设计的引物 sense GCAGCATTACCACAACTCACC antisense TTGCCTTTGCCCATTTCA 进行blast后得到如下结果 Score E Sequences producing significant alignments: (bits) Value gi|26000752|gb|AY161275.1| Oryctolagus cuniculus glucocorti... 42 4e-05 gi
4kOARRBoVVdV0du4Vtqi/f6tUqYIq26tIr4TAuH7dOly5dBnHbWyk45k6FDF06rRpMDIyitYy9pcjh4+AB2k+BEsVCrbGhQlkZgIsfGTm3U/i2n2ebMfe90CZuV1hSmcnUd9MqjDuOBiVVk3Fpa0X8bVhZyQ9qoA3zq06gCHlB6A42YH4OOzB0oPu0Kg7DWb50taH+e/fv2FDX1S7d1jh7v0HaEi/moebj0U9MiYVNhXxlSZNm0vCh7BJmEZfcqdOn6R
2F+DwTquooTcizAj6Sh40arj1e+iWJ2ispgsbT801bWi9OaaVDQ0u7N9Rf22Q7je3ypEkT/aXtAx/4gHh20RbetchuWXizve3YY/3L0LGHHqt8Cva+97zf/vEfvyYb4imWXpTxLz8m4rvgjodswc3X26Xnn2o3LbjZPv/Fv5GGuGa33/ZzO2n2HD17NAEWeUXwmQh44uy32U+uutL+69e+6jjedfsddvSxx3m8xNNFQ67wipqQKHyESZ35
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