Applications Key: W=Western Blotting IP=Immunoprecipitation Reactivity Key: H=Human M=Mouse R=Rat B=Bovine Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
TWEAK Receptor/Fn14 Antibody detects endogenous levels of total TWEAK Receptor/Fn14 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of the TWEAK Receptor/Fn14. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HT-1080, PC3 and HT29 cell lines, using TWEAK Receptor/Fn14 Antibody.
Background
The receptor for TWEAK, fibroblast growth factor-inducible 14 (Fn14), is a member of the TNF superfamily of receptors thought to be involved in cell growth, adhesion and migration (1). Its expression is regulated by a number of different growth factors (1,2). Elevated expression is also seen in some cancers including hepatocellular carcinomas (3), glioblastomas (4), and pancreatic cancer (5). The receptor contains a binding site for members of the TNFR-associated factor (TRAF) family that promotes the activation of NF-κB (1,6). Recent studies have suggeted some therapeutic potential of TWEAK and its receptor signaling in regards to autoimmunity and cancer treatment (7-9).