Phospho-Rb (Ser807/811) (D20B1

2) XP Rabbit mAb__®

价格询价

品牌Cell Signaling Technology
货号8516
产地USA
更新日期2026年05月28日

应用范围W, IP, IHC-P, IF-IC, F
适应物种H,M,R,Mk

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
Phospho-Rb (Ser807/811) (D20B12) XP Rabbit mAb__®
抗原：
synthetic peptide corresponding to residues surrounding Ser807/811 of human Rb protein
应用范围：
W, IP, IHC-P, IF-IC, F
库存：
大量
供应商：
CST
适应物种：
H,M,R,Mk
级别：
详见MSDS文件
保质期：
详见说明书
是否单克隆：
1
保存条件：
-20°c
规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	40 ul (4 western blots)	产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IF-IC F	H M R Mk	Endogenous	110	Rabbit IgG

Protocols	8516: IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity	Phospho-Rb (Ser807/811) (D20B12) XP^® Rabbit mAb recognizes endogenous levels of Rb protein only when phosphorylated at Ser807, Ser811, or at both sites. This antibody does not cross-react with Rb phosphorylated at Ser608.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser807/811 of human Rb protein. Western Blotting Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-Rb (Ser807/811) (D20B12) XP^® Rabbit mAb (upper) or Rb (4H1) Mouse mAb #9309 (lower). Western Blotting Western blot analysis of extracts from WI-38 cells, serum-starved for 3 days (-) or serum-starved for 3 days followed by treatment with 10% serum for 2 days (+), using Phospho-Rb (Ser807/811) (D20B12) XP^® Rabbit mAb. IP Immunoprecipitation of phospho-Rb (Ser807/811) from MCF7 cell extracts using Phospho-Rb (Ser807/811) (D20B12) XP^® Rabbit mAb (lane 2). Western blot was performed using the same antibody. Lane 1 is 10% input. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-Rb (Ser807/811) (D20B12) XP^® Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human lung carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-Rb (Ser807/811) (D20B12) XP^® Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human ovarian serous adenocarcioma using Phospho-Rb (Ser807/811) (D20B12) XP^® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). IF-IC Confocal immunofluorescent analysis of MCF7 (left) and BT549 (right) cells, untreated (top) or λ phosphatase-treated (bottom) using Phospho-Rb (Ser807/Ser811) (D20B12) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).
Background	The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9). Sherr, C.J. (1996) Science 274, 1672-7. Nevins, J.R. (1992) Science 258, 424-9. Welch, P.J. and Wang, J.Y. (1993) Cell 75, 779-90. Hu, Q.J. et al. (1990) EMBO J 9, 1147-55. Knudsen, E.S. and Wang, J.Y. (1997) Mol Cell Biol 17, 5771-83. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61. Connell-Crowley, L. et al. (1997) Mol Biol Cell 8, 287-301. Kitagawa, M. et al. (1996) EMBO J 15, 7060-9. Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
Application References	Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	8147 Phospho-Rb (Ser608) (D10F2) Rabbit mAb 8180 Phospho-Rb (Ser780) (D59B7) Rabbit mAb 9313 Rb (D20) Rabbit mAb 9309 Rb (4H1) Mouse mAb 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide 8112 SignalStain^® Antibody Diluent 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit) 5425 Normal Goat Serum 9997 Tris Buffered Saline with Tween 20 (TBST-10X) For Research Use Only. Not For Use In Diagnostic Procedures.