- 询价
- Cell Signaling Technology
- 2203
- USA
- 2026年05月28日
- W, IP, IHC-P, IF-IC, F
- H,M
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- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
TCF1 (C63D9) Rabbit mAb
- 抗原：
synthetic peptide corresponding to a region surrounding Pro95 of human TCF1 protein
- 应用范围：
W, IP, IHC-P, IF-IC, F
- 库存：
大量
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,M
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M | Endogenous | 48, 50 kDa | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | TCF1 (C63D9) Rabbit mAb detects endogenous levels of total TCF1 protein. This antibody does not recognize the dominant negative isoforms of TCF1 lacking the amino-terminal β-catenin binding domain and does not cross-react with LEF1. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro95 of human TCF1 protein. Western Blotting
Western blot analysis of total cell lysates from HCT29, Colo201, Jurkat and mouse thymocytes using TCF1 (C63D9) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using TCF1 (C63D9) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using TCF1 (C63D9) Rabbit mAb in the presence of control peptide (left) or TCF1 blocking peptide #1007 (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human tonsil using TCF1 (C63D9) Rabbit mAb. Flow Cytometry
Flow cytometric analysis of Jurkat cells using TCF1 (C63D9) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). IF-IC
Confocal immunofluorescent analysis of DLD-1 cells using TCF1 (C63D9) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). |
| Background | LEF1 and TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors that consists of the following: Lymphoid Enhancer Factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3, and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and act downstream in Wnt signaling. LEF1 and TCF bind to Wnt response elements to provide docking sites for β-catenin, which translocates to the nucleus to promote the transcription of target genes upon activation of Wnt signaling (3). LEF1 and TCF are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers including colon cancer (4,5). TCF1, also known as Transcription Factor 7 (TCF7), has several isoforms due to alternative splicing and transcription from an alternative promoter. The isoforms generated by the alternative promoter do not contain the amino-terminal β-catenin binding domain and therefore may function in a dominant negative manner (6). TCF1 displays dynamic expression both in the total amount and the type of isoforms expressed in T cells during development and differentiation (7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
过程中，一抗应在封闭缓冲液 (TBS/0.3%Triton. X–100/5% NGS) 中稀释。抗体：Phospho–Akt (Ser473) (D9E) XP. Rabbit mAb #4060、Phospho–EGFReceptor (Tyr1173) (53A5) Rabbit mAb #4407样本：石蜡包埋人类乳腺肿瘤（上图）和 HCC827 异种移植物（下图）抗体稀释：SignalStain. 抗体稀释液（左）或 TBST/5%NGS（右）检测—检测系统传统的 IHC 检测
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